Designing sequencing primers

Designing sequencing primers

Primer designing2GC for a 20mer2Absence of dimerization capability3Absence of significant hairpin formation usually 3 bp4Lack of secondary priming sites in the template5Low specific binding at the 3 end to avoid misprimingUsually a 2024 nt primer works wellOligo as well as other programs will pick decent primers the standard parameters used by such programs dont work with a given sequenceIn such a case primers need to be picked manuallyThe following hints should help in Source: Designing sequencing primers

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